PB4311 Protein and Protein Formulations Laboratory Syllabus:

PB4311 Protein and Protein Formulations Laboratory Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES

The course aims to
 Impart the knowledge of the formulation concepts on proteins.
 Evaluate the various protein and protein formulations through various characterization techniques.
 Expertise in various formulation approaches of proteins.

LIST OF EXPERIMENTS

 Preformulation study with suitable proteins and peptides
 Study on factors influencing solubility profile and partition coefficient of proteins.
 Compatibility and interactions studies of peptides and proteins with pharmaceutical excipients
 Stability study of protein and protein formulations
 Protein estimation using various methods.
 Isolation and estimation of DNA
 Isolation and estimation of RNA
 Estimation of Enzymatic activity.
 Prepare and characterize protein immobilized alginate beads.
 Quality control experiments with marketed protein and protein formulations
 Formulation and characterisation of vesicle based protein and peptide.
 Computational approaches in study of aggregation

LIST OF EQUIPMENTS REQUIRED

 UV Spectrophotometer
 pH Meter
 Analytical Weighing Balance
 Microscopes
 Stability Chamber
 Software for computational works to characterize the protein and protein formulations

TOTAL: 90 PERIODS

COURSE OUTCOMES:

On completion of the course, the student will be able to
1. Execute preformulation study on protein formulations.
2. Carryout the stability protocol on proteins.
3. Isolate and estimate DNA and RNA.
4. Formulate the various protein formulations.
5. Analyse the various quality control test on marketed formulations.
6. Become Expertise in the field of protein formulation discipline

REFERENCES

 Awesh Kumar Yadav, Rajeev Sharma, Atul Jain. “Practical Manual for M.Pharm. Students”, Nirali Prakashan, 2019.
 R. S. Sengar. Laboratory Manual of Biochemistry: Methods and Techniques. New India Publishing Agency, 2014.
 Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds.) Current Protocols in Molecular Biology. John Wiley & Sons, Inc. New York. 2003.
 Morriss-Andrews A, Shea J-E. Computational studies of protein aggregation: methods and applications. Annual Review of Physical Chemistry 2015(66):643–666
 Jeffrey L. Cleland, Robert Langer. “Formulation and Delivery of Proteins and Peptides”, Wiley VCH, 1994.
 Rakesh K. Tekade. “Dosage Form Design Parameters, Volume I”, Elsevier, Academic Press, 2018.