PB4212 Drug Discovery Laboratory Syllabus:
PB4212 Drug Discovery Laboratory Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
This course will explore the process of drug discovery from Synthetic and natural products .
SYNTHETIC METHODS FOR DRUG DISCOVERY
1. Synthesis of selected drugs involving two or more steps of synthesis and study of spectral analysis of drug synthesized (Paracetamol, Aspirin, Fluorscein, acetanilide, etc.).
2. Determination of pharmacopoeia standards for the synthesized drugs.
DISCOVERY OF DRUGS FROM NATURAL PRODUCTS
1. Extraction Techniques: Cold maceration, Hot Percolation and Soxhalation.
2. Evaluation of extraction Efficiency by yield calculation and TLC.
3. Isolation and purification of some of the following natural products
a. Piperine from black pepper
b. Strychnine and Brucine from Strychnos nuxvomica seeds
c. Caffeine from Tea Powder
d. Curcumin from Turmeric
e. Diosgenin from Diascoria tubers
f. Sennosides from Senna leaves
g. Embelin from Emblica ribes fruits
4. Identification of alkaloids in mixture by TLC.
5. Identification of phytoconstituents like alkaloids, steroids, flavanoids etc in plant extracts by TLC.
6. Separation (of sugars/amino acids) by paper chromatography.
7. Separation of compounds by HPLC
8. Analysis of recorded spectra of some simple organic compounds.
9. Tests to detect alkaloids, steroids, flavanoids and their glycosides.
10. Evaluation of antioxidant potential of herbal extracts using DPPH free Radicals cavenging assay.
TOTAL: 90 PERIODS
Required Equipments:
Soxhlet apparatus, rotary flash evaporator, Hot air oven, sonicator, mortar and pestle, TLC chamber, Fume hood, purification columns, micro-plate reader, UV spectrometer, centrifuge, required strains & consumables.
COURSE OUTCOMES:
On completion of this course students should be able to
1. describe the process of drug discovery and development.
2. discuss the challenges faced in each step of the drug discovery process .
3. have gained a basic knowledge of synthetic and extraction methods used in drug discovery.
4. organise information into a clear report.
5. demonstrate their ability to work in teams and communicate scientific information effectively.
6. perform common extraction techniques including maceration, percolation, soxalation etc.
REFERENCES
1. Foye’s Principles of Medicinal Chemistry. By David A. Williams, Thomas L.Lemke, Thomas L. Lernke, William O. Foye. Lippincott Williams& Wilkins Publishers; 7th Edition,2012.
2. Modern Methods of Plant Analysis – Peech and M. V. Tracey, 1955.
3. Natural Product Chemistry “A laboratory guide” by Raphealikan,2nd edition, 1991.
4. Phytochemistryvol I & II by Miller, Jan, Nostrant, Rein Hid, 2003.
5. Recent advances in Phytochemistry Vol. I & IV – Scilicet, Runeckles.
6. Remington: The Science and Practice of Pharmacy, 21st Edition, 2011.
7. Wilson and Gisvold’s Textbook of Organic Medicinal and Pharmaceutical Chemistry. ByJaime N. Delgado (Editor), Ole Gisvold (Editor), William A. Remers (Editor). Lippincott Williams& Wilkins Publishers; 10thEdition (August 1998) ISBN: 0397515839.1998