PB4202 Immuno Technology Syllabus:

PB4202 Immuno Technology Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

The course aims to
 understand the applications of immunology for the development of diagnostics
 understand the basic principles of vaccine development
 make use of the knowledge of immuno technology for clinical applications and also become aware of the regulatory issues.

UNIT I INTRODUCTION

Review on Cells of the immune system and their development; primary and secondary lymphoid organs; humoral immune response; cell mediated immune responses; complement, classification of T cells and B cells, cell markers.

UNIT II ANTIBODIES

Development of Monoclonal antibodies, classification and their applications; ELISA – types; IFT (direct and indirect) Agglutination tests; Antigen detection assay; Plaque Forming Cell Assay, Development of rapid immunodiagnostics – Immuno- lateral flow / flow through assays. Diagnosis of immediate and delayed hypersensitivity, anaphylactic reaction, total Ig and antigen specific IgE antibody assay, assay for hemolytic diseases, assay for immune complex, skin tests for DTH response

UNIT III DEVELOPMENT OF IMMUNOASSAYS

PBMC separation from the blood; identification of lymphocytes based on CD markers; FACS; Lympho proliferation assay; Mixed lymphocyte reaction; Cr51 release assay; macrophage cultures; cytokine bioassays- IL2, gamma IFN, TNF alpha.; HLA typing.

UNIT IV VACCINE TECHNOLOGY

Principles of vaccine development, types; Development of vaccines for bacterial, viral and parasitic diseases, Regulatory requirements for vaccine development and testing, ethical issues, protein based vaccines; sub-unit vaccines, DNA vaccines; Plant based vaccines; recombinant antigens as vaccines; reverse vaccinology, cancer vaccines, customized therapeutic cancer vaccines, (scFv) antibodies and molecular evolution of scFv for enhanced sensitivity and specificity

UNIT V DEVELOPMENT OF IMMUNOTHERAPEUTICS

Development of effective immuno drug targets for infectious diseases, engineered antibodies; catalytic antibodies; idiotypic antibodies; dendritic cells based immunotherapy, combinatorial libraries for antibody isolation, CAR T-cell therapy, Immune check point inhibitors.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

At the end of the course the students will be able to
 Understand the science of immuno technology
 Comprehend the concepts of antibodies and its characterization
 Describe the developments in the immunoassays
 Gain the knowledge of vaccine development
 Apply the technology for the development of immuno therapeutics and Diagnosis
 Become an entrepreneur in the field of immuno technology

REFERENCES:

1. Roitt, Ivan. Essential Immunology 9th Edition., Blackwell Scientific, 13th edition, 2017
2. Roitt I., Brostoff J. and Male D. Immunology, 6th ed. Mosby, 2001
3. Goldsby , R.A., Kindt, T.J., Osbome, B.A. and Kerby J. Immunology, 6th ed., W.H.Freeman, 2006
4. Janeway’s Immunobiology, Ninth Edition, Kenneth M. Murphy, Casey Weaver, 2017
5. Roitt’s Essential Immunology, 13th Edition, Peter J. Delves, Seamus J. Martin, Dennis R.Burton, Ivan M. Roitt, 2017
6. Lippincott Illustrated Reviews: Immunology, 2nd ed., 2012