PB4201 Protein and Protein Formulations Syllabus:

PB4201 Protein and Protein Formulations Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

 To provide the basic concepts of protein and protein formulations.
 To instill the principles of protein formulation and design
 To impart knowledge and skills necessary for knowing fundamental aspects of proteins and their formulations

UNIT I PROTEIN ENGINEERING

Concepts for protein engineering. Isolation and purification of proteins, Stability and activity based approaches of protein engineering, Chemical and Physical Considerations in Protein and Peptide Stability.

UNIT II PEPTIDOMIMETICS

Introduction, classification; Conformationally restricted peptides, design, pseudopeptides, peptidomimetics and transition state analogs; Biologically active template; Amino acid replacements; Peptidomimetics and rational drug design.

UNIT III PROTEOMICS

Protein identification and characterization: Methods/strategies, protein identification, denovo protein characterization, Isotope labelling, N- and C-terminal tags. 2-Dimensional gel electrophoresis Methods including immobilized pH gradients (IPGs), resolution, reproducibility and image analysis, future developments. Purpose of Protein glycosylation effect of protein glycosylation on the proteome

UNIT IV PROTEIN FORMULATION

Different strategies used in the formulation of DNA and proteins, Analytical and biophysical parameters of proteins and DNA in preformulation, Liposomes, PEGylation, Biological Activity, Biophysical Characterization Techniques, Forced degradation studies of protein.

UNIT V METHODS OF PROTEIN SEQUENCING

Various methods of protein sequencing, characterisation, Edman degradation, Tryptic and/or Chymotryptic Peptide Mapping.

TOTAL:45PERIODS

COURSE OUTCOMES

At the end of the course the students will be able to
 Understand the fundamentals of protein engineering.
 Discuss the underlying concepts of peptidomimetics and drug design.
 Demonstrate the characterization techniques for protein molecules.
 Incorporate approaches to formulate stable protein formulation.
 Elicit concepts of the protein sequencing.
 Become expertise in the technology of Protein and Protein Formulations

REFERENCES:

1. Eugene J. McNally, Jayne E. Hastedt Protein Formulation and Delivery, Informa Healthcare USA, Inc 2008
2. Engelbert Buxbaum, Fundamentals of Protein Structure and Function, Springer International Publishing Switzerland 2015
3. Ajay K. Banga Therapeutic Peptides and Proteins Formulation, Processing, and Delivery Systems. 3rd Edition, 2015. CRC Press, Taylor & Francis USA.
4. Sheldon J. Park, Jennifer R. Cochran, Protein Engineering and Design, 1ST Edition, 2009, CRC press. USA
5. Jeffrey L. Cleland, Rober Langer. Formulation and Delivery of Proteins and Peptides. ACS, USA.
6. Robert K. Skopes. Protein purification, principles and practice, springer, New york.
7. David Whitford, Proteins-Structure and Function, 1st Edition, 2005. John Wiley & Sons, USA.
8. Lars Hovgaard, Sven Frokjaer, Marco van de Weert. Pharmaceutical Formulation Development of Peptides and Proteins. 2nd Edition, CRC Press, Taylor & Francis, USA.