PB4103 Industrial Fermentation Syllabus:
PB4103 Industrial Fermentation Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
To enable the students to understand the concepts of fermentation technology applied to industrial processes for making products: fermenters, reaction kinetics, media formulation, utilization of microbial cultures, design aspects of bioreactors.
UNIT I INTRODUCTION TO BIO REACTOR DESIGN AND CONSTRUCTION
Bioreactor selection criteria and classification, Parameters for control, Design of ideal reactors, Single (Batch, Flow) and multiple reactors, Non-Ideal flow, RTD studies, Modelling of Non-ideal flow reactors, Design and operation of various bioreactors, viz CSTF, fed batch systems, air-lift bioreactors, fluidized bed bioreactors, Scale-up studies.
UNIT II MICRO BIALKINETICS AND DESIGN OF VARIOUS CULTIVATION PROCESSES
Immobilized and solid state cultivation; Kinetics of growth in batch continuous and fed batch culture. Specific growth rate, doubling time, growth yield, metabolic quotient; stoichiometry balance equation, carbon- nitrogen balance, oxidation – reduction principles, product formation. Biomass productivity, comparison with batch cultures, residual time distribution, test of validity; product formation, total cell retention cultivation, .Effect of inhibitors and
activators on growth.
UNIT III MODELING OF RECOMBINANT CULTIVATION ANIMAL AND PLANT CELL CULTIVATION SYSTEMS FOR THE RAPEUTIC PROTEINS
Structuredmodelsofmetabolismandgrowth,modelsofgeneexpressionandregulation,ageneralized model of plasmid replication, Genetic instability, predicting host-vector interactions and genetic instability. Process considerations for utilizing genetically engineered strains. Media, aeration in cell culture systems, Bioreactors for plant/animal suspension culture, cell immobilization and organized tissue, bioreactor considerations for animal/plant cell culture for production of pharmaceuticals, The rapeuticproteins and Monoclonalanti bodies.Industrial application softhebioreactorsas cell cultivation systems. Introduction to cell banking and storage – .mammalian, insects, stem cells and Microbes.
UNIT IV DOWNSTREAM PROCESSING AND SEPARATION TECHNIQUES
Characteristics of biological materials: Recovery and purification of fermentation products; pretreatment methods; Separation of cell mass: centrifugation, clarification and filtration; removal of host cells proteins (HCP) and viral plasmids proteins, viral inactivation. Modern techniques: Electrophoresis; Chromato graphic methods; Membrane processes- Ultrafiltration; Reverse osmosis; Cross flow filtration; Microfiltration; Isoelectric focusing; Affinity based separations. Advantages and disadvantages of the above methods.
UNIT V CASE STUDIES INFERMENTATION DERIVED PRODUCTS
Case studies on Whole cell immobilization and their industrial application. Production of penicillin, recombinant Insulin, amino acids-lysine and glutamic acid. Enzymes -amylase and protease. Case studies should deal with strain improvement, medium design, reactor design and process optimization etc.
SELF STUDY TOPICS (NOT FOR EXAMINATIONS):
Drying, Drying curve, Batch and continuous dryers, Case studies for the separation of intracellular and extracellular
products, Evaporation and crystallization.
TOTAL: 45PERIODS
COURSE OUTCOMES:
After completion of the course, a student will be able to achieve these outcomes
1. Apply the knowledge of fermentation technology in industrial processes
2. Handle and utilize microbial systems for biological reactions for making products
3. Design and use of reactor systems for bioprocesses.
4. Analyse kinetics of cell and product formation in batch, continuous and fed-batch cultures
5. Differentiate the rheological changes during fermentation process
6. Detail the downstream process of fermentation of important microbial products.
REFERENCES
1. Stanbury,Stephen.P.F.,Hall,J.and Whitaker,A.“Principles of fermentation technology” Elsevier 3rd edition.
2. Bailey, J.E. and Ollis, D.F. “Biochemical Engineering Fundamentals” 2nd Edition.,McGrawHill,1986.
3.B.Sivashankar,“Bioseparationprinciplesandtechniques”.PrenticeHallofIndiaPvtLtd2007 4Blanch,H.WandClarkD.S.,“BiochemicalEngineering”,MarcelDekker,1997
5. Doran,PaulineM,“BioprocessEngineeringPrinciples”.AcademicPress,1995
6. Nielsen,J.andVilladsen,J.“BioreactionEngineeringPrinciples”.Springer,2007.
7. Shuler,M.L.and Kargi, F. “Bioprocess Engineering: Basic Concepts”. 2nd Edition,Prentice-Hall,2002.