PB4102 Pharmaceutical Microbiology Syllabus:
PB4102 Pharmaceutical Microbiology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
Upon successful completion of this course the student will be able to
Understand the mechanism of action of chemotherapeutic and antibiotic resistence.
Identify the microorganisms of relevance to healthcare and the pharmaceutical industry and their sources.
obtain the knowledge on microbial contamination/product spoilage and antimicrobial preservation in pharmaceutical formulations
UNIT I CHEMOTHERAPEUTIC AGENTS
Introduction to chemotherapeutic agents: History and development of chemotherapeutic agent, Properties of antimicrobial agents, Types of chemotherapeutic agents – Synthetic, Semisynthetic, Natural. Antibiotics: Types of antibiotics with their mode of action; antibacterial, antifungal, antiviral, antiprotozoal
UNIT II ANTIBIOTIC RESISTANCE AND DEVELOPMENT OF NEW THERAPEUTICS
Antibiotic resistance: Development of antibiotic resistance amongst pathogens Mechanism of antibiotic resistance, Disease management methods. Antimicrobial Peptides: History, properties, sources, mode of action, application. Plant based therapeutic agents. Different prophylactic and therapeutic methods in control of infections. Introduction to virology.
UNIT III CLEAN ROOM TECHNOLOGY AND BIOSAFETY REGULATIONS
Microbial contamination spoilage and hazard Sources of contamination, factors affecting survival and growth. Methods of sterilizations: Steam, dry heat, Radiation, Gaseous and Filtration. Clean room technology and its regulatory aspects. Principles of sterilizations with respect to pharmaceutical industries. Biosafety regulations- BSL-1, BSL-2, BSL-3 and BSL4.
UNIT IV PRESERVATION OF PHARMACEUTICAL PRODUCTS
Principles of preservation: objectives of preservation, the ideal preservative, rational development of a product preservative system etc. Antimicrobial preservatives and their properties: antimicrobial activity, factors affecting antimicrobial activity, preservative monographs. Preservative stability and efficacy. Evaluation methods of Preservative and testing.
UNIT V MICROBIAL FERMENTATIONS
Industrial importance of microbes in the production of Enzymes- Amylase, Invertase, Proteolytic. Vaccines -Recombinant and Synthetic Vaccines. Organic acids-citric acid, acetic acid, and α- keto glutaric acid. Bio-insecticides-Bacillus sp., Baculovirus. Antibiotics Penicillin, Bacitracin, Streptomycin. Vitamins-Vitamin B12, Vitamin A, Riboflavin.
SELF STUDY TOPICS (NOT FOR EXAMINATIONS):
Role of microbes in the degradation of pollutants / toxic compounds, Microbial waste products in the market.
REFERENCES:
1. Hugo, WB and Russell, AD. Pharmaceutical Microbiology, (2003). Blackwell Science, Oxford, UK.
2. Geoffrey Hanlon and Norman Hodges. Essential Microbiology for pharmacy and pharmaceutical science. (2013).Wiley Blackwell.
3. S. P. Vyas & V. K. Dixit. Pharmaceutical Biotechnology. (2003) CBS Publishers & Distributors, New Delhi.
4. Prescott’s Microbiology 8th Edition by Willey, Joanne, Sherwood, Linda, Woolverton, Chris.
5. Davis, B. D., Dulbecco, R, Eisen, H. N., Ginsberg, R. S. Microbiology. (1990). Harper and Row Publishers, Singapore.
6. L.E. Casida, Industrial microbiology, New Age International Publishers.2005