PB4101 Analytical Techniques in Pharmaceutical Biotechnology Syllabus:
PB4101 Analytical Techniques in Pharmaceutical Biotechnology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
To enable the students To have a fundamental knowledge about the molecular spectroscopy, NMR and Mass spectroscopy
To acquire knowledge on different chromatographic methods for separation of pharmaceutical and biotechnological products.
To acquire knowledge about the principles and operations of various modern analytical instruments.
UNIT I MOLECULAR SPECTROSCOPY
UV-Visible spectroscopy- Theory, Laws, Instrumentation, and applications; IR spectroscopy: Theory, Molecular vibrations, Sample handling, Instrumentation of Dispersive and Fourier – Transform IR Spectrometer and Applications; Spectro flourimetry: Theory of Fluorescence, Factors affecting fluorescence, Quenchers, Instrumentation and Applications Atomic absorption spectroscopy: Principle, Instrumentation, Interferences and Applications, Case studies on applications of molecular spectroscopy.
UNIT II NMR SPECTROSCOPY
Quantum numbers and their role in NMR, Principle, Instrumentation, Solvent requirement in NMR, Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, Principles of 1H-NMR and 13C NMR. Pharmaceutical and biological applications of NMR spectroscopy.
UNIT III MASS SPECTROSCOPY
Principle, Theory, Instrumentation, Types of ionization like electron impact, chemical, field, FAB and MALDI, APCI, ESI, APPI, Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Pharmaceutical and biological applications of Mass spectroscopy, Case studies on biological applications of mass spectroscopy.
UNIT IV CHROMATOGRAPHY
Analytical & Preparative concepts of – Principle, instrumentation, chromatographic parameters, factors affecting resolution and applications of High-performance Thin Layer chromatography, Ion exchange chromatography, Column, Gas, High Performance Liquid chromatography and Affinity chromatography, Case studies on usage of ion exchange, HPLC and affinity chromatography in industrial applications.
UNIT V ELECTROPHORESIS & IMMUNOASSAYS
Principle, Instrumentation, working conditions, factors affecting separation and applications of the following: Gel electrophoresis, Capillary electrophoresis, Zone electrophoresis, Iso electric focusing; RIA (Radio immuno assay), ELISA and Bioluminescence assays; Analysis of host cell protein (HCP) and host cell DNAs (HCD) in biopharmaceuticals, viable cell analysis, measurement of energy metabolism of live cell, metabolic analyzer – principle and instrumentation.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
On completion of the course, students will able to
1. Understand the fundamental principles and applications of UV-visible, IR, flame emission, atomic absorption, NMR and Mass spectroscopy
2. Demonstrate the principles and applications of chromatographic and electrophoretic separation techniques
3. Recognize the importance of modern instruments in the pharmaceutical analysis
4. Apply the theoretical knowledge of instruments for new analytical method development in screening of various pharmaceutical agents.
5. Develop ability to involve in chemical and biological standardization of pharmaceutical products.
6. Assess appropriate techniques for the analysis of various pharmaceuticals and biotechnological products.
REFERENCES:
1. Spectrometric Identification of Organic compounds – Robert M Silverstein, Sixth edition, John Wiley & Sons, 2004.
2. Principles of Instrumental Analysis – Doglas A Skoog, F. James Holler, Timothy A. Nieman, 5th edition, Eastern press, Bangalore, 1998.
3. Instrumental methods of analysis – Willards, 7th edition, CBS publishers.
Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th edition, CBS Publishers, New Delhi, 1997.
4. Organic Spectroscopy – William Kemp, 3rd edition, ELBS, 1991.
5. Quantitative Analysis of Drugs in Pharmaceutical formulation – P D Sethi, 3rd Edition, CBS Publishers, New Delhi, 1997.
6. Pharmaceutical Analysis- Modern methods – Part B – J W Munson, Volume 11, Marcel Dekker Series