PB4007 Bioconjugate Technology Syllabus:

PB4007 Bioconjugate Technology Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

 At the end of the course, the student would have learnt about enzymes, nucleic acids and how to modify them for target specificity. Student also gets familiarized with the industrial applications of this technology.

UNIT I FUNCTIONAL TARGETS

Modification of Amino Acids, Peptides and Proteins – Modification of sugars, polysaccharides and glycoconjugates – modification of nucleic acids and oligonucleotides.

UNIT II CHEMISTRY OF ACIVE GROUPS

Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxylatereactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and ketone reactive chemical reactions – Photoreactive chemical reactions.

UNIT III BIOCONJUGATE REAGENTS

Zero length cross linkers – Homobifunctional cross linkers – Heterobifunctional crosslinkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.

UNIT IV ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION

Properties of common enzymes – Activated enzymes for conjugation – biotinylatedenzymes – chemical modification of nucleic acids – biotin labeling of DNA- enzyme conjugation to DNA – Fluorescent of DNA.

UNIT V BIOCONJUGATE APLICATIONS

Preparation of Hapten-carrier Immunogen conjugates – antibody modification and conjugation – immunotoxin conjugation techniques – liposome conjugated and derivatives- Colloidal – gold-labeled proteins – modification with synthetic polymers.

TOTAL : 45 PERIODS

COURSE OUTCOMES:

At the end of the course the student will be able to,
1. Acquire the knowledge of modifying functional agents such as proteins, sugars, etc.,
2. Apply the knowledge of chemical reactivity of active groups
3. Know about the conjugate reagents
4. Attain the knowledge of enzyme and nucleic acids modification and conjugation
5. Have the knowledge of bioconjugate applications
6. Apply the knowledge of bioconjugate and its applications

REFERENCES:

1. Chemistry of bioconjugates : synthesis, characterization, and biomedical applications / edited by Dr. Ravin Narain, Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada.
2. Hermanson, G.T. “Bioconjugate Techniques”. Academic Press 3rd edition, 2013.
3. Sam Massa and Nick Devoogdt (eds.), Bioconjugation: Methods and Protocols, Methods in Molecular Biology, vol. 2033, Springer Science Business Media, LLC, part of Springer Nature 2019.
4. Sonny S. Mark (ed.), Bioconjugation Protocols: Strategies and Methods, Methods in Molecular Biology vol. 751, DOI 10.1007/978-1-61779-151-2_1, Springer Science+Business Media, LLC 2011.
5. Chrostof M.Niemeyer (Eds) Methods in Molecular Biology. 283. Bioconjugation Protocols Strategies and Methods. Humana Press.