PB4006 Bioprocess Engineering and Technology Syllabus:
PB4006 Bioprocess Engineering and Technology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
Gain knowledge about the design of production of bioproducts under aerobic and anaerobic states, process economic and preparation of flow sheet of production process.
State the enzyme kinetics, various factors regulating catalysis, different models for analyzing the enzyme kinetics, Immobilization and large-scale production of enzyme
UNIT I BLACK BOX MODEL
Yield coefficients, black box stoichiometries, elemental balances, heat balance, degrees of reduction balances, systematic analysis of black box stoichiometries, and identification of gross measurement errors
UNIT II DESIGN OF FERMENTATION PROCESSES
Kinetics of substrate utilization, biomass growth and product formation, inhibition of cell growth and product formation. Design and operation of continuous cultures, chemostat in series, batch and fed batch cultures, total cell retention cultivation.
UNIT III MODELING OF VARIOUS FERMENTATION PROCESSES
Principles of model building for biotechnological processes, unstructured models on the population level, structured models on the cellular level, morphologically structured model, genetically structured models, cybernetic model, modeling of recombinant systems.
UNIT IV BIOREACTOR DESIGN & CONSTRUCTION
Basic design and construction of CSTR, bioreactor design of agitator / agitator motor, power consumption in aerated bioreactor, design of sparger, mixing time estimation, oxygen mass transfer capability in bioreactor, Removal of Heat in bioreactor, Main parameters to be monitored and controlled in fermentation processes.
UNIT V CASE STUDIES IN FERMENTATION DERIVED PRODUCTS
Case studies on Production of green chemicals, algal biofuels, recombinant Insulin. Case studies on medium design, reactor design & process optimization.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
Upon successful completion of this course the students will be able to:
1. Develop the capacity of production processes and control of aerobic and anaerobic systems, solve calculation based on process economy as well as to recognize the importance of flow sheet of the production system
2. Explain the kinetics of enzyme catalysed reaction in free and immobilized states.
3. organise the production of microbial enzymes and operate variables affecting the production process.
4. demonstrate about concept and criteria of scale up of laboratory process, Instrumentation and process control- offline and online.
5. Collect the proficient knowledge of translation of lab data to pilot level, they will be able to solve features involved in the scale up process, process monitoring and control.
6. plan a research career or to work in the biotechnology industry with strong foundation about bioreactor design and scale-up.
REFERENCES:
1. Shuler, M.L., Kargi F., “Bioprocess Engineering – Basic Concepts “, Prentice Hall, 2nd Edition, 2015.
2. Pauline D., “Bioprocess Engineering Principles “. Elsevier, 2nd Edition, 2012.
3. Nielsen, J. and Villadsen, J. “Bioreaction Engineering Principles”. Springer, 3rd Edition, 2011.
4. Lydersen B.K., “Bioprocess Engineering Systems, Equipment and Facilities” , Wiley Blackwell, 2nd Edition, 2010.
5. Bailey, J.E. and Ollis, D.F. “Biochemical Engineering Fundamentals”, 2nd Edition, McGrawHill, 2017.
6. Stanbury, P.F., Stephen J.H., Whitaker A., “Principles of Fermentation Technology”, Science & Technology Books, 2nd Edition, 2009.