NT4006 Nano Biophotonics Syllabus:
NT4006 Nano Biophotonics Syllabus – Anna University PG Syllabus Regulation 2021
OBJECTIVES:
To learn about Fundamentals of light and optics
To study the concepts of optical based imaging techniques.
To learn about recent development in optical sensors.
UNIT I BASICS OF LIGHT AND OPTICS
Interaction of light with cells, tissues, non-linear optical processes with intense laser beams, photoinduced effects in biological systems.
UNIT II IMAGING TECHNIQUES
Light microscopy, wide-field, laser scanning, confocal, multiphoton, fluorescence lifetime imaging, FRET imaging, Frequency-Domain lifetime imaging. Cellular Imaging, Imaging of soft and hard tissues and other biological structures.
UNIT III SINGLE MOLECULE SPECTROSCOPY
UV-VIS spectroscopy of biological systems, single molecule spectra and characteristics –IR and Raman spectroscopy and Surface Enhanced Raman Spectroscopy for single molecule applications.
UNIT IV OPTICAL FORCE SPECTROSCOPY
Generation optical forces – Optical trapping and manipulation of single molecules and cells in optical confinement – Laser trapping and dissection for biological systems – single molecule biophysics, DNA protein interactions.
UNIT V SENSORS AND OPTICAL TECHNIQUES
Biosensors, fluorescence immuoassay, flow cytometry, Fluorescence correlation spectroscopy, Fluorophores as cellular and molecular tags.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
CO1: Students will gain knowledge in basics of optics
CO2: Students will gather idea about imaging techniques
CO3: Students will acquire information about Biophotonics and advanced optical sensors
REFERENCES:
1. Laser Tweezers in Cell Biology in Methods in Cell Biology, Vol.55, Michael P. Sheetz(Ed.), Academic Press 1997.
2. P.N. Prasad, Introduction to Biophotonics, John-Wiley, 2003.
3. G. Marriot & I. Parker, Methods in Enzymology, Vol.360,2003.
4. G. Marriot & I. Parker, Methods in Enzymology, Vol.361,2003.