BY4312 Bioprocess and Downstream Processing Laboratory Syllabus:

BY4312 Bioprocess and Downstream Processing Laboratory Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

1 The course applies earlier learned knowledge about mass transfer in bio reactors and sterilization kinetics.
2 To provide hands on training in Downstream processing through simple experimentations in the laboratory.
3 To understand the nature of the end product, its concentration, stability and degree of purification required for targeted biological products.

LIST OF EXPERIMENTS

1. Enzyme immobilization studies – Gel entrapment, adsorption and cross linking immobilization.
2. Batch cultivation – E.coli – growth rate, substrate utilization kinetics, product analysis after induction, metabolite analysis by HPLC.
3. Fed batch cultivation – E.coli – growth rate, substrate utilization kinetics, product analysis after induction, metabolite analysis by HPLC.
4. Continuous cultivation – x – D construction, kinetic parameter evaluation, gas analysis, carbon balancing.
5. Optimization techniques – Plackett Burman, Response surface methodology.
6. Bioreactor studies: Sterilization kinetics, kLa determination, residence time distribution.
7. Cell separation methods-Centrifugation and microfiltration
8. Cell disruption methods- ultrasonicator, homogeniser.
9. Aqueous two phase extraction of biologicals.
10. Protein precipitation by salting –out method (ammonium sulphate).
11. Protein purification method- Column chromatography.
12. Product polishing- dryers, crystallizers.

LIST OF EQUIPMENTS REQUIRED

Centrifuge,
Column for purification
Ultrasonicator
Homogeniser
Microfiltration capsule
Hot air oven, Incubator
Laminar air flow chamber
HPLC,
CHEMICALS All required chemicals & stains.

TOTAL : 90 PERIODS

OUTCOMES:

After completion of the course the students will be able to
CO1 Gain ability to investigate, design and conduct experiments, analyze and interpret data, and apply the laboratory skills to solve complex bioprocess engineering problems.
CO2 Acquire knowledge for the separation of whole cells and other insoluble ingredients from the culture broth.
CO3 Learn the basic principles and techniques of chromatography to purify the biological products and formulate the products for different end uses.

REFERENCES

1. J.C. Janson – Protein Purification – Principles, High Resolution Methods And Applications, 3rd Edition, Wiley, 2011.
2. Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications
3. Shuler and Kargi, “ Bioprocess Engineering “, 3rd Edition, Prentice Hall, 2017.