BY4251 Metabolic Process and Engineering Syllabus:
BY4251 Metabolic Process and Engineering Syllabus – Anna University PG Syllabus Regulation 2021
OBJECTIVES:
To familiarize the students with a quantitative basis, for the analysis of cellular metabolism
To understand the metabolic networks in single cells and at the organ level
To enable the students to use organisms to produce valuable substances on an industrial scale in cost effective manner
To know how to optimize strategy for directed genetic changes in microbes for better strain production
UNIT I CELLULAR METABOLISM
Transport Processes – Fueling reactions – Glycolysis, fermentative pathways – TCA cycle and oxidative phosphorylation, anaplerotic pathways –Catabolism of fats, organic acids, and aminoacids – Biosynthesis of aminoacids, nucleic acids, and fatty acids – Polymerization – Growth energetics.
UNIT II REGULATION, MANIPULATION AND SYNTHESIS OF METABOLIC PATHWAY
Regulation of enzyme activity – Regulation of enzyme concentration – Regulation of metabolic networks – Regulation at the whole cell level – Metabolic pathway manipulations – Enhancement of Product yield and productivity – Extension of substrate range, product spectrum and novel products (Antibiotics, Polyketides, Vitamins) – Improvement of cellular properties – Metabolic pathway synthesis algorithm – Lysine biosynthesis.
UNIT III ANALYSIS AND METHODS FOR THE METABOLIC FLUX
Metabolic flux map – Fluxes through the catabolic pathways in microbes– Metabolic flux analysis for determined, over-determined and under-determined systems –Sensitivity analysis – Direct flux determination from fractional label enrichment – Applications involving complete enumeration of metabolite isotopomers – Carbon metabolite balances-GC-MS for metabolic flux analysis – genome wide technologies
UNIT IV GENOME BASED METABOLIC MODEL DEVELOPMENT
Development of Genomic scale metabolic model, Insilico Cells: studying genotype-phenotype relationships using constraint-based models, case studies in E. coli, S.cerevisiae metabolic network reconstruction methods, optimization of metabolic network, Identification of targets for metabolic engineering; software and databases for genome scale modeling
UNIT V ANALYSIS OF METABOLIC CONTROL AND INDUSTRIAL CASE STUDIES
Fundamental of Metabolic Control Analysis (MCA), MFA, and MPA and their application, Multisubstrate enzyme kinetics, Metabolic engineering examples for bio-fuel, bio-plastic and green chemical synthesis , Study of genome scale model in various systems for the production of green chemicals using software tools
TOTAL: 45 PERIODS
OUTCOMES:
After completion of metabolic engineering, students will be able
To learn stoichiometry and energetics of metabolism.
To apply practical applications of metabolic engineering in chemical, energy, medical and environmental fields.
To have a clear understanding on metabolic control analysis
To gain experience in the development of genome scale metabolic modelling
To integrate modern biology with engineering process to meet desired needs
REFERENCES
1. Christiana D. Smolke, “ The Metabolic Pathway Engineering Handbook Fundamentals”, CRC Press Taylor & Francis Group, 2010.
2. Cortossa, S., Aon, M.A., Iglesias, A.A. and Lloyd.D., “An Introduction to Metabolic and Cellular Engineering”, 2nd Edition, World Scientific Publishing Co, 2011
3. Curran, C.P., “Metabolic Processes and Energy Transfers – An Anthology of Current Thought”, The Rosen Publishing group, Inc., 2006.
4. Nielsen, J., Villadsen, J. and Liden, G., “Bioreaction Engineering Principles”,3rdEdition,Springer, 2011
5. Stephanopoulos, G.N., Aristidou, A.A. and Nielsen.J., “Metabolic Engineering – Principles and Methodologies”, Elsevier Science, 2001.