BY4201 Bio Separation Technology Syllabus:
BY4201 Bio Separation Technology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES
1 To enable the students to understand the methods to obtain pure proteins, enzymes and in general about product development R & D
2 To gain depth knowledge and hands on experience on Downstream processes to commercial therapeuticaly important proteins.
3 To educate the principle involved in membrane separations and enrichment operations
4 To promote the applicability of chromatographic techniques in Biological products separation
5 To educate about the finishing operations and formulations of commercial applications.
UNIT I DOWNSTREAM PROCESSING IN BIOTECHNOLOGY
Role and importance of downstream processing in biotechnological processes – Problems and requirements of bio product purification – Economics of downstream processing in Biotechnology, cost-cutting strategies – Separation characteristics of proteins and enzymes – size, stability, properties – Flocculation and conditioning of broth – Process design criteria for various classes of bio products (high volume, low value products and low volume, high value products) – Upstream production methods affect downstream purification strategies.
UNIT II PHYSICO-CHEMICAL BASIS OF BIO-SEPARATION PROCESSES
Cell disruption methods for intracellular products – Physical, chemical, mechanical – Removal of insoluble, biomass and particulate debris separation techniques – Filtration at constant pressure and at constant rate – Empirical equations for batch and continuous filtration – Types of filtration – Centrifugal and cross – flow filtration – Types of filtration equipments – Centrifugation – Basic principles, design characteristics – Types of ccentrifuges and applications – Sedimentation
UNIT III MEMBRANE SEPARATIONS AND ENRICHMENT OPERATIONS
Theory, Design consideration and configuration of membrane separation processes – Reverse osmosis, microfiltration, ultra filtration, dialysis and pervaporation – Structure and characteristics of membranes – Membrane modules – Enrichment Operations – Extraction–equipment for extraction – Aqueous two-phase extraction process – Evaporators – Types of evaporators – Adsorption isotherms and techniques – Protein precipitation – Methods of precipitation.
UNIT IV MECHANISM AND MODES OF CHROMATOGRAPHIC SEPARATION
Chromatography – Classification of chromatographic techniques – General description of column chromatography – Chromatographic terms and parameters – Practice of chromatography – Partition, normal-phase, displacement, reversed-phase, size exclusion, ion exchange, hydrophobic, affinity chromatography – Scale-up of chromatography – Process considerations in Preparative liquid chromatography and HPLC .
UNIT V FINISHING OPERATIONS AND FORMULATIONS
Drying – Mechanism, methods and applications, Types of dryers – Tray, spray, rotary, belt, disc – Crystallization – Nucleation , growth – Types of crystallizers – Tank, scrapped surface, Oslo, Circulating-magma evaporator – Freeze drying – Principle, process, applications – Case studies Citric acid, Penicillin , Cephalosporin, Recombinant Streptokinase, Interferon.
TOTAL : 45 PERIODS
COURSE OUTCOMES
After completion of the course the students will be able to
CO1 Understand the methods to obtain pure proteins, enzymes and in general about product development R & D
CO2 Gain depth knowledge and hands on experience on Downstream processes to commercial therapeuticaly important proteins.
CO3 Understand the principle involved in membrane separations and enrichment operations
CO4 Understand the applicability of chromatographic techniques in Biological products separation
CO5 Understand the importance of finishing operations and formulations of commercial applications.
REFERENCES
1. Belter, P.A., Gussler, E.L. and Hu, W.S., “Bio-separation: Downstream Processing for Biotechnology”, John Wiley and Sons, 2011.
2. Forciniti, D., “Industrial Bio-separation: Principles & Practice”, Blackwell, 2008.
3. Ghosh, R., “Principles of Bio-separations Engineering”, World Scientific Publishers, 2006.
4. Ladisch, M.R., “Bioseparations Engineering: Principles, Practice, and Economics”, John Wiley & Sons, 2001.
5. Roger, H., “Bio-separations Science and Engineering”, Oxford University Press, 2006.