BY4111 Immuno Technology Laboratory Syllabus:

BY4111 Immuno Technology Laboratory Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES

1. To give practical exposure in the clinical diagnosis.
2. To give laboratory training in different immune technological techniques.
3. To give hands on training in cell staining, separation and identification

LIST OF EXPERIMENTS

1. *Preparation of antigen and Routes of immunization (Intraperitoneal, Sub-cutaneous, Intramuscular, Intra- nasal, Oral – VIRTUAL DEMO)
2. *Methods of bleeding (Tail bleeding, Intravenous, intraorbital – VIRTUAL DEMO)
3. Collection of serum, storage and purification of total IgG (salt precipitation).
4. Evaluation of Antibody titre by direct ELISA
5. Evaluation of Antigen by Sandwich ELISA
6. Characterization of antigens by native and SDS-PAGE
7. Characterizations of antigens by Western blot analysis – Wet and semidry transfer
8. Conjugation of Immunoglobins (Streptavidin, colloidal gold)
9. Methods for prototype development of Immunodiagnostics (ICT card)
10. Blood smear identification of leucocytes by Giemsa stain
11. Separation of mononuclear cells by Ficoll-Hypaque
12. Separation of splenocytes and proliferation against mitogens
* Approval of IAEC is mandatory for experiments involving Live animals

TOTAL : 90 PERIODS

LIST OF EQUIPMENTS REQUIRED

Microscopes,
Purification columns,
Microplate reader,
UV spectrometer,
PAGE apparatus,
Western blot apparatus,
Centrifuge,
Haemocytometer

COURSE OUTCOMES

After completion of the course the students will be able to
1. Understand the clinical diagnosis methods and its importance.
2. Gain training in different immunotechnological techniques.
3. Gain hands on training in cell staining, separation and identification