BY4007 Bio Reactor Design and Control Syllabus:
BY4007 Bio Reactor Design and Control Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES
1 To familiarize various reactor types and modes of operations.
2 To incorporate mass transfer engineering into bioprocess system
3 To impart knowledge on the selection and design of bioprocess equipment
4 To integrate the engineering concepts in to scale up and scale down of bioreactor
5 To impart knowledge on the bioreactor instrumentation and control
UNIT I BASIC BIOREACTOR CONCEPTS
Bioreactor Operation – Batch operation, semi-continuous and fed-batch operation, Continuous Operation – Chemostat, turbidostat – Microbiological reactors, enzyme reactors – Tank-type, Column-type biological reactors – Case studies – Continuous Fermentation with Biomass Recycle, Tanks-in-series, Tubular plug flow bioreactors.
UNIT II AERATION AND AGITATION IN BIOPROCESS SYSTEMS
Mass transfer in agitated tanks – Effect of agitation on dissolved oxygen – Correlations with kLa in Newtonian and non Newtonian liquid – Power number, Power requirement for mixing in aerated and non aerated tanks for Newtonian and non Newtonian liquids – Agitation rate studies – Mixing time in agitated reactor, residence time distribution – Shear damage, bubble damage, Methods of minimizing cell damage – Laminar and Turbulent flow in stirred tank bioreactors.
UNIT III SELECTION AND DESIGN OF BIOPROCESS EQUIPMENT
Materials of construction for bioprocess plants – Design considerations for maintaining sterility of process streams processing equipments, selection, specification – Design of heat and mass transfer equipment used in bioprocess industries – Requirements, design and operation of bioreactor for microbial, plant cell and animal cell.
UNIT IV SCALE UP AND SCALE DOWN ISSUES
Effect of scale on oxygenation, mixing, sterilization, pH, temperature, inoculum development, nutrient availability and supply – Bioreactor scale-up based on constant power consumption per volume, mixing time, impeller tip speed (shear), mass transfer co-efficients – Scale up of downstream processes – Adsorption (LUB method), Chromatography (constant resolution etc.), Filtration (constant resistance etc.), Centrifugation (equivalent times etc.), Extractors (geometry based rules) – Scale–down related aspects.
UNIT V BIOREACTOR INSTRUMENTATION AND CONTROL
Bioreactor controlling probes – Characteristics of bioreactor sensors – Methods of measuring process variables –Temperature – Flow measurement and control – Pressure measurement and control – Agitation – shaft power, rate of stirring – Detection and prevention of foam – Measurement of Microbial biomass – Measurement and control of Dissolved oxygen – Inlet and outlet gas analysis – pH measurement and control – Biosensors.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
At the end of the course, the students will be able
CO1 To select appropriate bioreactor configurations and operation modes based upon the nature of bio products and cell lines and other process criteria.
CO2 To understand the aeration and agitation in bioprocess systems
CO3 To understand the selection and design of bioprocess equipment
CO4 To apply modelling and simulation of bioprocesses so as to reduce costs and to enhance the quality of products and systems
CO5 To integrate research lab and Industry; identify problems and seek practical solutions for large scale implementation of Biotechnology
REFERENCES
1. Impre, J.F.M.V., Vanrolleghem, P.A. and Iserentant, D.M., “Advanced Instrumentation, Data Interpretation and Control of Biotechnological Processes”, Kluwer Academic Publishers, 2010.
2. Mann, U., “Principles of Chemical Reactors Analysis & Design: New tools for Industrial Chemical Reactor Operations”, Willey–VCH, 2009.
3. Mansi, E.M.T.EL., Bryce, C.F.A., Demain, A.L. and Allman, A.R., “Fermentation Microbiology and Biotechnology”, 3 rd edition Taylor and Francis, 2012.
4. Towler, G. and Sinnott, R., “Chemical Engineering Design: Principles, Practice, Economics of Plant and Process Design”, 2 nd edition, Butterworth – Heinemann ltd., Elsevier, 2012.