BY4004 Phytochemistry Syllabus:

BY4004 Phytochemistry Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

1 To give the details of plant derived value added compounds and its functions .
2 To educate the importance of phytocompounds application to fight against various microbial infections
3 To provide knowledge on biotech based production of agro medicines separation techniques
4 To educate about the basic principles and techniques involved in structure elucidation and Molecular Modeling
5 To provide knowledge on secondary metabolites and its role in development of herbal therapeutics

UNIT I INTRODUCTION TO PHYTOCHEMISTRY

Phytochemicals and their classification–Phytochemical screening –Physiochemical tests – Macroscopic and microscopic techniques –Traditional plant and Herbal remedies –– Herbal drugs WHO guidelines–Standardization of Herbal Drugs Derivatives with Special Reference to Brazilian Regulations

UNIT II PHYTOCOMPOUNDS

Plant extract used to Bacterial, Fungal and Parasitic infection – Biological and Toxicology Properties of plant extract –Anti-MRSA and Anti-VRE activities of Phytoalexins and Phytoncides– Anti microbial and targeted screening of Plant extract – Plant derived compound against drug resistant microorganisms – Antioxidant and antitumor Plant metabolites (fruits and vegetables)– Bioactive compounds as food

UNIT III PHYTOMEDICINE

Medicinal Plants for Development of Phytomedicine and Use in Primary Health Care– Immuno stimulants and adaptogen from Plants –Polyphenols for Atherosclerosis and Ischemic Heart disease –Cancer Chemopreventive agents –Lipidoxidation nitrogen Radicals– Phytochemicals in oilseeds – Flavonoids in Cardiovascular disease – Bioengineering and Breeding approaches in improving phytochemical content of plants.

UNIT IV SEPARATION TECHNIQUES AND STRUCTURE ELUCIDATION

Thin layer chromatography– HPTLC– Column chromatography – GC-MS – LC-MS –HPLC – Partition chromatography – Gas chromatography – FT-IR – UV- NMR (1D&2D) – X-ray diffraction – QSAR and Molecular Modeling

UNIT V SECONDARY METABOLITE

Secondary metabolite production through cell culture system–Hairy root induction–Methods of gene transfer–Chemical methods– PEG – dextran–Physical method– Electroporation– Microinjection– Lipofection delivery for herbal therapeutics–Quality Control –Germplasm improvement.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

After completion of the course the students will be able to
CO1 Understand the importance the plant derived value added compounds and its functions .
CO2 Understand the importance of phytocompounds application to fight against various microbial infections
CO3 Develop depth knowledge on biotech based production of agro medicines separation techniques
CO4 Understand the basic principles and techniques involved in structure elucidation and Molecular Modeling
CO5 Develop depth knowledge on secondary metabolites and its role in development of herbal therapeutics

REFERENCES

1. Ahamed, I., Aqil, F. and Owais, M.“Modern Phytomedicine”, Turning medicinal Plants into drugs. WILEY VCH, Verlag GmbH & Co, KGaA, Weinheim. 2006.
2. Arnason, J.T., Arnason, J.E. and Arnason, J.T.,“Phytochemistry of Medicinal Plants”, Kluwer Academic Publishers, 1995.
3. Bidlack, W.R., Omaye, S.T., Meskin, M.S.and Topham, D.K.W.,” Phytochemicals as Bioactive Agents”, 1St Edition, CRC Press, 2000.
4. Meskin, M.S., Bidlack, W.R., Davies, A.J. and Omaye, S.T., “Phytochemicals in Nutrition and Health”, CRC Press, 2002.
5. Rasooli, I, “Bioactive compounds in Phytomedicine” , Intech Open access Publishers , 1st Edition, 2011
6. Durgesh Nandini Chauhan and Kamal Shah ,”Phytopharmaceuticals Potential Therapeutic Applications”,Scrivener publishing,Wiley,2021