BY4001 Advances in Animal Biotechnology Syllabus:
BY4001 Advances in Animal Biotechnology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVE:
1 To educate the students about the scope, regulatory issues and commercially available products produced using of animal biotechnology.
2 To provide depth knowledge about the available viral vectors that can be used to create recombinant DNA for gene therapy purposes so that they can undertake research /project work related to biopharming.
3 To teach the importance of cell culture study for invitro study purposes and for scaling up the products at commercial level.
4 To provide depth knowledge about creation of recombinant products for gene therapy purpose and the importance of molecular probe which is an important tool for medical and forensic studies.
5 To educate the principle behind invitro fertilization and biopharming in order to create transgenic animal of commercial importance.
UNIT I INTRODUCTION
Scope of Animal Biotechnology, Animal Biotechnology for production of regulatory proteins, blood products, vaccines, hormones and other therapeutic proteins.
UNIT II MOLECULAR BIOLOGY
Biology of animal viral vectors- SV40, adeno virus, retrovirus, vaccinia virus, herpes virus, adeno associated virus and baculo virus. Applications of commercially available viral vectors and their pros and cons
UNIT III CELL CULTURE TECHNOLOGY
Culturing of cells, primary and secondary cell lines, Cell culture-Scaling up of animal cell culture monolayer culture, suspension culture; Various bio-reactors used for animal cell culture-Roller bottle culture; Bioreactor process control, stirred animal cell culture, Air-lift fermentor, hemostat/Turbidostat; High technology vaccines; Hybridoma technology; Cell lines and their applications
UNIT IV GENETIC ENGINEERING
Gene therapy-prospects and problems, Recent advancements in Gene therapy; Knock out mice and mice model for human genetic disorder; Baculo virus in biocontrol; Enzymes technology, Somatic manipulation of DNA, Nucleic acid hybridization and probes in diagnosis- preparation of probes, evaluation and applications. Recent advancements in diagnostic tool development and its diagnostic procedure
UNIT V ADVANCEMENTS AND APPLICATIONS
Rumen manipulation- probiotics embryo transfer technology, invitro fertilization, transgenesis methods of transferring genes into animal oocytes, eggs, embryos and specific tissues by physical, chemical and biological methods; Biopharming –Transgenic animals (case study : Mice, Cows, Pigs, Sheep, Goat, Birds and Insects); Artificial insemination and embryo transfer.
TOTAL : 45 PERIODS
COURSE OUTCOMES:
After completion of the course the students will be able to:
CO1 Understand the scope, regulatory issues and commercially available products produced using of animal biotechnology.
CO2 Gain knowledge about the available viral vectors that can be used to create recombinant DNA for gene therapy purposes so that they can undertake research /project work related to biopharming.
CO3 Understand the importance of cell culture study for invitro study purposes and for scaling up the products at commercial level.
CO4 Gain knowledge in creating recombinant products for gene therapy purpose and the importance of molecular probe which is an important tool for medical and forensic studies.
CO5 Understand the principle behind invitro fertilization and biopharming in order to create transgenic animal of commercial importance.
REFERENCES:
1. Watson, J.D., Gilman, M., WitowskiJ. and Zoller, M. Recombinant DNA, 3rd ed., Scientific American Books, 2007
2. Glick, B.R. and Pasternack, J.J. Molecular Biotechnology, 3rd ed., ASM Press, 2003
3. Lewin, B. Genes VIII , Pearson Prentice Hall, 2004.
4. Davis J.M. Basic Cell Culture: A Practical Approach, IRL Press, 2nd ed., 2002
5. Freshney R.I. Animal Cell Culture- a practical approach, 6th ed., 2010