BO4311 Modern Methods of Pharmaceutical Analysis Laboratory Syllabus:

BO4311 Modern Methods of Pharmaceutical Analysis Laboratory Syllabus – Anna University PG Syllabus Regulation 2021

OBJECTIVE:

 To carry out analytical experiments related to spectroscopic and chromatographic techniques.
 The analysis of various drugs in single and combination dosage forms.
 Theoretical and practical skills of the instruments.
 Method development and validation for the analysis of drugs in pharmaceutical formulations.

LIST OF EXPERIMENTS

1. Calibration of volumetric glasswares.
2. Establishing standard operating procedure (SOP) and Calibration records for analytical balance, pH meter and UV/Vis spectroscopy.
3. Determination of λmax.
4. Effect of change in physio-chemical parameters on absorbance spectrum of a drug molecule.
5.Quantitative and qualitative analysis of drug molecule using standard comparison method by UV/Vis spectroscopy and HPLC.
6. Quantitative analysis of drug molecule using E1%1cm method by UV/Vis spectroscopy.
7. Quantitative analysis of drug molecule using calibration graph method by UV/Vis spectroscopy and HPLC.
8. Separation and identification of mixtures of drugs by TLC.
9. Separation and identification of amino acids by paper chromatography.
10. Identification of functional group of a drug molecule by IR spectroscopy.
11. Determination of impurities by limit test.
12. Quantitative analysis by titrimetric methods.

TOTAL: 90 PERIODS

LIST OF EQUIPMENT FOR BATCH OF 30 STUDENTS

1. Digital weighing balance
2. Digital pH meter
3. UV chamber
4. TLC chamber
5. UV/Vis spectroscopy
6. HPLC
7. IR spectroscopy

OUTCOMES:

Student will be able to perform,
1. * Preparation and standardization of various assay reagents with respect to chemical and
drug
analysis.
2. * Separation and quantification of drugs molecules by chromatographic and spectral
techniques.

REFERENCES:

1. Atherden L.M, “Bentley and Driver’s Textbook of Pharmaceutical Chemistry”, 8th Edition, Oxford University Press, 2004.
2. Siddiqui, Anees A, “Pharmaceutical Analysis”. Vol.I& II, 3rd edition, CBS Publishers, 2014.
3. Takeru Higuchi, Einar Brochmann, Hanffen Hanssen, Hamffen Hanssen, “Pharmaceutical Analysis” 1st Edition, CBS Publishers, 2005.
4. Loyd V. Allen Jr, “Remington: The Science and Practice of Pharmacy”. Vol. I & II, 22nd Edition, Pharmaceutical Press;, 2012.
5. Kenneth A. Connors, “Text book of Pharmaceutical Analysis”, 3rd Edition, John wiley and sons, New York,2007