BO4211 Immunopharmacology Laboratory Syllabus:
BO4211 Immunopharmacology Laboratory Syllabus – Anna University PG Syllabus Regulation 2021
OBJECTIVES
The course aims to,
provide hands-on-experience on handling animal for research and various relevant immunological techniques like ELISA, Flow cytometry etc.
provide practical experience on performing and understanding immunoassays forevaluating drugs and vaccines.
To provide practical exposure in the clinical diagnosis.
To provide laboratory training for different immunotechnological techniques.
To give hands on training in cell staining, separation and identification.
LIST OF EXPERIMENTS
1. Selection and Handling of animals used in immunopharmacological assays (Eg. Mice, Rat, Rabbit, Zebra fish, Caenorhabditis elegans etc.,).
2. Preparation of antigens and immunization procedures for raising anti-sera.
3. Demonstration of various methods of bleeding, serum separation and storage.
4. Antibody titre by ELISA method (Indirect ELISA).
5. Sandwich ELISA – Quantification of antigens.
6. Immunoprecipitation/Immuno electrophoresis.
7. Isolation and purification of IgG from serum (Ammonium sulphate method/Protein A).
8. Studies for characterisation of antigens – SDS -PAGE, Immunoblotting, Dot blot assays.
9. Assay for immunostimulants (Erythropoietin assay etc., ).
10. Direct Agglutination-Widal test, Salmonella detection.
11. Separation of mononuclear cells by Ficoll-Hypaque.
12. Separation and culturing of splenocytes and demonstration of T cell proliferation.
13. PBMC proliferation/cell viability by mitogen/antigen by MTT or Thymidine uptake assay.
14. Flow Cytometry- Identification of lymphocytes and their subsets.
15. Evaluation of monoclonal antibodies for diagnostic and therapeutic applications.
16. Demonstration of Immunodiagnostics using commercial kits (Rapid Flow through and Lateral flow devices– Dot Blot and StripTest).
TOTAL : 90 PERIODS
COURSE OUTCOMES
At the end of the course the student will be able to,
CO1 learn the principles of immunoassays employed in academic research.
CO2 learn how to perform the immunopharmacological assays used in the evaluation of vaccines and immunotherapeutics.
CO3 learn how to handle methods of planning and performing immunological techniques required in industries.
REFERENCES
1. “Antibodies”, Cold Spring Harbour Laboratory, 1988.
2. Goldsby, R.A. et al.“Kuby Immunology”. 6 thEdition, W.H. Freeman, 2002.
3. Turgeon, Mary Louise. “Immunology and Serology in Laboratory Medicine”, 2nd Edition, Elsevier, 2007.
4. Brostoff J et al., “Clinical Immunology”, 6th Edition, Gower Medical Publishing, 2002.
5. Coligan, J. E. et al, “Current Protocols in Immunology”, 4thEdition John Wiley & Sons, 1994.
6. Paul, “Fundamental of Immunology”, 4thEdition, Lippincott Raven, 1999.