BO4018 Fermentation Technology Syllabus:
BO4018 Fermentation Technology Syllabus – Anna University PG Syllabus Regulation 2021
OBJECTIVE
The subject provides knowledge involving basic principle of fermentation process, microbial kinetics and recombinant protein production along with case studies, to help the students understand fermentation processes involved in Pharmaceutical Industries.
To acquire knowledge on down streaming process.
To design and construct bioreactor for various applications.
To acquire knowledge on different separation techniques.
To acquire knowledge on microbial kinetics.
UNIT I INTRODUCTION TO BIOREACTOR DESIGN AND CONSTRUCTION
General requirements Basics of fermentation processes- range and component parts, functions of a fermenter. Basic design and construction of CSTR, bioreactor design of agitator/agitator motor, power consumption in aerated bioreactor, design of sparger, mixing time estimation, oxygen mass transfer capability in bioreactor, achievement of aseptic conditions, Removal of Heat in bioreactor, Main parameters to be monitored and controlled in fermentation processes.
UNIT II MICROBIAL KINETICS AND DESIGN OF VARIOUS CULTIVATION PROCESSES
Overview of batch, continuous and fed batch cultures and comparison between the underlying concepts. Simple unstructured kinetic models for microbial growth of bacterial, fungal, animal and plant systems, kinetics of substrate utilization, biomass growth and product formation in continuous cultures, batch and fed batch cultures, total cell retention cultivation, inhibition on cell growth and product formation.
UNIT III MODELING OF RECOMBINANT CULTIVATIONANIMAL AND PLANT CELL CULTIVATION SYSTEMS FORTHERAPEUTIC PROTEINS
Structured models of metabolism and growth, models of gene expression and regulation, a generalized model of plasmid replication, Genetic instability, predicting host-vector interactions and genetically instability. Process considerations for utilizing genetically engineered strains. Media, aeration in cell culture systems, Bioreactors for plant/animal suspension culture, cell immobilization and organized tissue, bioreactor considerations for animal /plant cell culture for production of pharmaceuticals, Therapeutic proteins and Monoclonal antibodies. Industrial applications of the bioreactors as cell cultivation systems.
UNIT IV DOWNSTREAM PROCESSING AND SEPARATION TECHNIQUES
Characteristics of biological materials: Recovery and purification of fermentation products; pretreatment methods; Separation of cell mass: centrifugation, clarification and filtration; Different methods of cell disruption; Solid shear method and liquid shear method; Different concentration methods: evaporation, distillation, crystallization, evaporation, SCFE, solvent extraction, phase separation, drying etc., whole broth extraction, protein precipitation; extraction; adsorption; Modern techniques: Electrophoresis; Chromatographic methods; Membrane processes- Ultrafiltration; Reverse osmosis; Cross flow filtration; Microfiltration; Isoelectric focusing; Affinity based separations. Advantages and disadvantages of the above methods.
UNIT V CASE STUDIES IN FERMENTATION DERIVED PRODUCTS
Case studies on Production of penicillin, recombinant Insulin. Case studies should deal with strain improvement, medium design, reactor design and process optimization etc.
TOTAL: 45 PERIODS
OUTCOME
This course work will made the students to:
CO1 apply the knowledge of design and construction of bioreactor in fermentation technology
CO2 Acquire the knowledge on microbial kinetics and biomass growth
CO3 design and use the reactor systems for bioprocesses
CO4 Analyse the various separation techniques and its application principles in the downstream processing
CO5 Expand and update the current fermentation technology information by using recent case studies
CO6 Acquire the knowledge and become expert in the field of field of fermentation technology
REFERENCES
1. Stanbury, Stephen. P. F., Hall, J. and Whitaker, A. “Principles of fermentation technology” Elsevier 3rd edition.
2. Bailey, J.E. and Ollis, D.F. “Biochemical Engineering Fundamentals” 2nd Edition., McGrawHill,1986.
3. B.Sivashankar, “Bio separation principles and techniques”. Prentice Hall of India Pvt Ltd 2007
4 Blanch, H.W and Clark D.S., “Biochemical Engineering”, Marcel Dekker,1997
5. Doran, Pauline M, “Bioprocess Engineering Principles”. Academic Press, 1995
6. Nielsen, J. and Villadsen, J. “Bioreaction Engineering Principles”. Springer, 2007.
7. Shuler, M.L. and Kargi, F. “Bioprocess Engineering: Basic Concepts”. 2nd Edition, Prentice-Hall, 2002.