BO4007 Modern Methods of Pharmaceutical Analysis Syllabus:
BO4007 Modern Methods of Pharmaceutical Analysis Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVE:
To enable students to acquire knowledge in various advanced analytical techniques used in the screening of pharmaceutical agents.
UNIT I UV-VISIBLE SPECTROSCOPY
Brief introduction of spectroscopy, EMR and principle of absorptions by molecule. The absorption law – Beer’s and Lambert’s law, limitations and Chromophores and their interaction with EMR, Theory of electronic transition theory, choice of solvent and solvent effects, modern instrumentation – design and working principle. Applications of UV-Visible spectroscopy (various qualitative and quantitative methods), Woodward – Fischer rules for calculating absorption maximum.
UNIT II IR SPECTROSCOPY AND THERMAL METHODS OF ANALYSIS
Infrared radiation, theory of IR absorption by a molecule, vibrational frequency and factors influencing vibrational frequency, rotational degrees of freedoms, transmission/absorption modes, types of bands, instrumentation and sampling techniques, interpretation of spectra, applications in pharmaceuticals. FT-IR-theory and applications, Attenuated Total Reflectance (ATR).Instrumentation and applications of thermal methods – Thermo Gravimetric Analysis (TGA),Differential Scanning Calorimetry (DSC), Differential Thermal Analysis (DTA) and Thermo Mechanical Analysis (TMA).
UNIT III NUCLEAR MAGNETIC RESONANCE PECTROSCOPY
Basic theory of NMR/PMR, excitation/emission process and instrumentation. solvents, reference compound, scale of measurement, shielding/deshielding; chemical shift, and factors affecting chemical shift, spin-spin coupling, coupling constant, and factors influencing the value of coupling constant, spin-spin decoupling and shift reagents, proton exchange reactions, FT- NMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in pharmaceuticals, spectral interpretations, 13C NMR, Natural abundance and applications.
UNIT IV MASS SPECTROMETRY
Basic principles, instrumentation and ionization methods; precursor ion/product ion production and fragmentation pattern; atmospheric pressure ionization (API), Chemical ionization (CI), Field Ionization (FI), Fast Atom Bombardment (FAB), Matrix assisted laser desorption ionization(MALDI), Time of Flight (TOF), hybridization with other techniques, and interpretation of mass spectrum and applications in pharmaceuticals.
UNIT IV CHROMATOGRAPHIC METHODS
Classification of chromatographic methods on mechanism of separation: High Performance Liquid Chromatography : Principle, instrumentation, solvents, packing materials and applications in pharmaceuticals; Gas Chromatography: principle, theory, column operations, instrumentation, derivatisation methods and applications in pharmaceuticals; HPTLC and Super Critical Fluid Chromatography (SFC): Theory, instrumentation, elution techniques and pharmaceutical applications; Principles, classifications, instrumentation, moving boundary electrophoresis, Zone Electrophoresis (ZE), Iso-electric focusing (IEF) and applications.
TOTAL: 45 PERIODS
COURSE OUTCOME:
CO1. Understand the fundamental principles and applications of UV-visible, IR, flame emission, atomic absorption, NMR and Mass spectroscopy
CO2. Demonstrate the principles and applications of chromatographic and electrophoretic separation techniques
CO3. Recognize the importance of modern instruments in the pharmaceutical analysis
CO4. Apply the theoretical knowledge of instruments for new analytical method development in screening of various pharmaceutical agents.
CO5. Develop ability to involve in chemical and biological standardization of pharmaceutical products.
CO6. Assess appropriate techniques for the analysis of various pharmaceuticals and biotechnological products
REFERENCES
1. “Chromatographic Analysis of Pharmaceuticals”, John A. damovics, 2nd edition, 1996.
2. “HPTLC – Quantitative Analysis of Pharmaceutical Formulations”– P. D. Sethi,1990.
3. “Identification of Drugs and Pharmaceutical Formulations by Thin LayerChromatography”– P.
D. Sethi, Dilip Charegaonkar, 2nd Edition, 2014.
4. “Instrumental Methods of Analysis”– Hobert H. Willard, 7th Edition, 1992.
5. “Instrumental Methods of Chemical Analysis”– B. K. Sharma – 9th Edition,2000.
6. “Liquid Chromatography – Mass Spectrometry”, W.M.A.Niessen, J. Van Der Greef, Vol.58,2006.
7. “Organic Chemistry” by I.L.Finar Vol. II – 5thedition, 1956
8. “Organic Spectroscopy”– William Kemp, 3rd Edition, 1991.
9. “Pharmaceutical Analysis – Modern Methods”– Part A, Part B, James W.Munson–2001.
10. “Practical Pharmaceutical Chemistry”, Part II, A. H. Beckett &J. B. Stenlake, 4th Edition,2015.
11. “Principles of Instrumental Analysis” by Donglas A. Skoog, James, J. Leary, 4th Edition,1992.
12. “Spectrometric Identification of Organic Compounds”, Robert. M. Silverstein Et Al, 8th edition, 2014.
13. “Spectroscopy of Organic Compounds” by P. S. Kalsi, 2007.
14. “Techniques and Practice of Chromatography”– Raymond P. W. Scott, Vol. 70,2003.
15. “Vogel’s Text Book of Quantitative Chemical Analysis”, 6th Edition, 2004.