BO4005 Advances in OMICS Sciences and Technology Syllabus:
BO4005 Advances in OMICS Sciences and Technology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
The course aims to,
Provideadvancedtheoreticalknowledgeontheorganizationandfunctionof genome
Understand the principles of functional genomic analyses
Have knowledge on the advanced methods and approaches in proteomics.
UNIT I MICROARRAYS IN GENOMICS
Microarrays, types, Designing and production of microarrays; cDNA microarray technology; Oligonucleotide arrays; Sample preparation, labeling, hybridization, generation of microarray data. Transcriptomics using cDNA and oligonucleotide arrays.
UNIT II NEXT GENERATION SEQUENCING TECHNOLOGIES
Overview of Next Generation Sequencing (NGS) technologies; Principles of NGS by Roche/454, Illumina, Life Technologies, Pacific Biosciences, Ion Torrent technologies; Applications of NGS to disease diagnosis and personalized medicine.
UNIT III PROTEIN MICROARRAYS AND YEAST TWO-HYBRID SYSTEM
Types of protein arrays; Protein microarray fabrication; Experimental analysis of proteins arrays. Data acquisition and processing; Applications of protein microarray types. Principles and methods in yeast two-hybrid system, Advances in yeast two hybrid system and its applications.
UNIT IV TWO-DIMENSIONAL GEL ELECTROPHORESIS OF PROTEINS
Sample preparation, First-dimension IEF with IPG; Second dimensional separation of proteins; Image analysis of 2-DE gels; DIGE, Protein expression profiling and comparative proteomics of complex proteomes using 2-DE.
UNIT V MASS-SPECTROMETRY
Basics of Mass-spectrometry (MS) and bimolecular analysis; Common ionization methods for peptide/protein analysis; Principles of Time of Flight (TOF), Ion Trap (IT), and Orbitrap mass analyzers; Mass spectrometry based proteomics: MALDI-TOF, Nano-LC-MS; Gas- chromatography coupled to Mass spectrometry; Mass-spectrometry analysis of Post-Translational Modifications of proteins.
TOTAL :45 PERIODS
COURSEOUTCOMES:
At the end of the course, the student will be able to,
CO1. Understand the designing and application of microarrays in genomics.
CO2. Have knowledge in next generation sequencing technologies and their use in diagnosis and personalized therapy.
CO3. Have exposure to protein analysis using high end technology
CO4. Acquire the knowledge of 2D gel Electrophoresis of proteins
CO5. Understand the concepts of mass spectrometry in protein analysis
REFERENCES:
1. Schena M. (2000) DNA Microarrays – A Practical Approach. Oxford University Press.
2. Rinaldis E. D. and Lahm A (2007) DNA Microarrays. Horizon bioscience.Causton,H.C.
3. Muller H. J. and Roder T. (2006) Microarrays. Elsevier Academic Press.
4. Causton H. C., Quackenbush J., and Brazma A. (2004) A Beginner’s Guide.
5. Schena M. (2005) Protein Microarrays. Jones and Bartlett Publishers.
6. O’Connor C. D. and Hames B. D. (2008) Proteomics. Scion Publishing Ltd.
7. Hoffman E. D. and Stroobant V. (2007) Mass Spectrometry – Principles and Applications, John Wiley & Sons Ltd.