BO4009 Microbial Technology Syllabus:
BO4009 Microbial Technology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVE:
To provide fundamental knowledge of pharmaceutical microbiology and microorganisms associated with the manufacture of pharmaceuticals
To study the importance of Microorganisms in Industry
To study the Structure and function of cell and cell communication
To gain knowledge on Cell culture technology and its applications in pharmaceutical industries.
To gain knowledge on Microbial pathogenesis and correlating it to rational use of antimicrobial agents.
UNIT I BIOLOGY OF MICROORGANISMS
Introduction – Microscopy – Structure and form of the bacterial cell – size, shape and structure of the cell wall and cytoplasmic membrane – Appendages to the bacterial cell – Capsules and slime – Bacterial spore – process of spore formation – Germination of spores – Toxins produced by bacteria – Yeasts and moulds – Introduction – Structure- Cell wall – Properties of selected fungi – Saccharomyces cerevisiae, Neurospora crassa, Penicillium, Aspergillus, Epidermophyton, Microsporum and Trichophyton
UNIT II INFECTIOUS DISEASES
Introduction – Spread of infection – Common source infections – Principles of microbial pathogenicity and epidemiology – Properties of selected pathogens – Staphylococcus, Streptococcus, Neisseria, Clostridium, Listeria, Pseudomonas, Vibrio, Yersinia, Haemophilus, Escherichia, Salmonella, Shigella, Proteus, Helicobacter- Chlamydia, Rickettsia, Mycobacterium – Spirochetes – Borrelia, Treponema and Leptospira, Candida and Cryptococcus
UNIT III ANTIBIOTICS AND OTHER ANTIMICROBIAL AGENTS
Antibiotics – definition, sources and types of antibiotics – penicillins, cephalosporins – Lincomycins, Tetracyclines, Rifamycins and Macrolides – Structure- activity relationships – Pharmacokinetic properties – Antifungals – synthetic antimicrobial agents – Mechanism of action – Bacterial resistance to antibiotics – Antivirals – Methisazone, nucleoside analogues – interferons – Clinical uses of antimicrobial agents
UNIT IV MICROBIAL ASPECTS OF PHARMACEUTICAL PROCESSING
Ecology of microorganisms as it affects the pharmaceutical industry – Microbial spoilage and preservation of pharmaceutical products – Contamination of non-sterile pharmaceuticals in hospitals and community Environments – Principles and practice of sterilization – Sterilization control and sterility assurance – Sterile pharmaceutical products – Factory and hospital hygiene and good manufacturing practice.
UNIT V BIOCATALYST TECHNOLOGY
Advantages and disadvantages of biocatalysis over chemical catalysis; Different types of biocatalysis: Microbial, enzymatic and immobilized system of biocatalysis; Current industrial biocatalysis; Biocatalysis with different enzymes: Lipase, amidase/ aminopeptidase, Acylase, Hydantoinase, lyases, Oxidoreductase, Nitrilase, Epoxide hydrolase, Hydroxylase, Aldolases, Decarboxylase
TOTAL : 45 PERIODS
COURSE OUTCOME:
The students would have learnt various aspects of pharmaceutical microbiology include:
CO1. the research and development of anti-infective agents
CO2. the use of microorganisms to detect mutagenic and carcinogenic activity in prospective drugs,
CO3. the use of microorganisms in the manufacture of pharmaceutical products.
REFERENCES:
1. Ching T. Hou. Handbook of Industrial Bio catalysis. CRC Press, 2019.
2. Frank Austen, K., Burakoff, S.J., Fred Rosen, Terry B. Strom, Therapeutic Immunology, Blackwell Science, Boston, 3rd Edition, 2006.
3. Hugo, W.B. and Russell, A.D. Pharmaceutical Microbiology – 8th Edition – Wiley-Blackwell, 2011
4. Mims C.A. The Pathogenesis of Infectious Disease, 6th Edition – London: Academic Press, 2015.
5. Thomas J. Kindt, Barbara A. Osborne, Richard A. Goldsby, Kuby Immunology, W.H., Freeman & Co, San Francisco, 6th Rev. Edition, 2006.