BO4008 Protein Engineering and Industrial Applications Syllabus:

BO4008 Protein Engineering and Industrial Applications Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVE:

 To provide the basic concepts of protein and protein formulations.
 To instill the principles of protein formulation and design
 To impart knowledge and skills necessary for knowing fundamental aspects of proteins and their formulations
 To provide advanced knowledge of proteins and their structure function relationship, essential for future pharmaceutical technology.

UNIT I INTRODUCTION

Amino acids, primary structure of proteins, amino acid composition, industrial significance, primary structure determination by chemical methods including automated sequencing and by gene sequencing, significance of primary structure determination, peptide synthesis, secondary structure and super secondary structures

UNIT II PROTEIN ARCHITECTURE

Tertiary structure of proteins, types of proteins, domains, quaternary structure, protein complexes, protein-protein interactions

UNIT III STRUCTURE-FUNCTION RELATIONSHIP

DNA-binding proteins: prokaryotic transcription factors, Helix-turn-Helix motif in DNA binding, Trp repressor, Eucaryotic transcription factors, Zn fingers, helix-turn helix motifs in homeodomain, Leucine zippers Membrane proteins: General characteristics, Transmembrane segments, prediction, bacteriorhodopsin and Photosynthetic reaction center Immunoglobulins: IgG Light chain and heavy chain architecture, Abzymes and Enzymes: Serine proteases, understanding catalytic design by engineering trypsin, chymotrypsin and elastase, substrate assisted catalysis other commercial applications.

UNIT IV PROTEIN ENGINEERING METHODS

Protein engineering methods, amino acid side chain reactions, chemical modification of proteins, site directed mutagenesis, posttranslational modifications and engineering.

UNIT V INDUSTRIAL APPLICATIONS OF PROTEIN ENGINEERING

Examples of industrial protein engineering applications Engineering of serine proteases, engineering of antibodies, engineering of proteins for thermal stability, engineering of proteins for preventing aggregation, His-tagged proteins in purification, engineering proteins for secretion, de novo protein synthesis.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

On completion of the course, students will learn to:
1. Understand the fundamentals of protein engineering.
2. Discuss the underlying concepts of peptidomimetics and drug design.
3. Demonstrate the characterization techniques for protein molecules.
4. Incorporate approaches to formulate stable protein formulation.
5. Elicit concepts of the protein sequencing.
6. Become expertise in the technology of Protein and Protein Formulations

REFERENCES:

1. Alberghina, L. “Protein Engineering in Industrial Biotechnology”. Harwood Academic Publications, 2000.
2. Branden C. and Tooze J., “Introduction to Protein Structure”, 2nd Edition, Garland Publishing,1999.
3. Creighton, T.E. “Proteins: Structure and Molecular Properties”, 2nd Edition, W.H.Freeman, 1993
4. Holland, I Barry et al., “ABC Proteins: From Bacteria to Man”. Academic Press Elsevier, 2003.
5. Moody P.C.E. and Wilkinson A.J. “Protein Engineering”. IRL Press, Oxford, 1990.
6. Rees, A.R., Sternberg, M.J.E. and Wetzel, R. “Protein Engineering: A Practical Approach”. IRL Press, 1992
7. Voet, D. and Voet, G., “Biochemistry”. 4th Edition, John Wiley and Sons, 2001.
8. Whitford, David “Proteins: Structure and Function”. John Wiley & Sons, 2005.