BO4002 Bioconjugate Technology and Applications Syllabus:

BO4002 Bioconjugate Technology and Applications Syllabus – Anna University PG Syllabus Regulation 2021

COURSE OBJECTIVES:

The course aims to,
 Provide advanced theoretical knowledge on Bioconjugate technologies.
 Learn about the biological and clinical applications of bioconjugate technology

UNIT I MODIFICATION OF FUNCTIONAL TARGETS

Modification of amino acids, peptides and proteins – modification of sugars, polysaccharides and glycoconjugates – modification of nucleic acids and oligonucleotides.

UNIT II CHEMISTRY OF ACTIVE GROUPS

Amine reactive chemical reactions – Thiol reactive chemical reactions – carboxyl reactive chemical reactions – hydroxyl reactive chemical reactions – aldehyde and ketone reactive chemical reactions – Photoreactive chemical reactions.

UNIT III BIOCONJUGATE REAGENTS

Zero length cross linkers – Homo bifunctional crosslinkers – Hetero bifunctional cross linkers – Trifunctional cross linkers – Cleavable reagent systems – tags and probes.

UNIT IV ENZYME AND NUCLEIC ACID MODIFICATION AND CONJUGATION

Properties of common enzymes – Activated enzymes for conjugation – biotinylated enzymes– chemical modification of nucleic acids – biotin labeling of DNA- enzyme conjugation to DNA – Fluorescent of DNA.

UNIT V BIOCONJUGATE APPLICATIONS

Preparation of Hapten-carrier Immunogen conjugates – antibody modification and conjugation – immunotoxin conjugation techniques – liposome conjugated and derivatives-Colloidal – gold labeled proteins – modification with synthetic polymers.

TOTAL: 45 PERIODS

COURSE OUTCOMES:

At the end of the course the student will be able to,
CO1 Understand target bio-molecules target and their active groups for conjugation.
CO2Get knowledge about different types of bio-conjugate reagents.
CO3 Have exposured to conjugation of enzymes, antibody and nucleic acid and the application of the conjugated products.

REFERENCES:

1. Chemistry of bioconjugates : synthesis, characterization, and biomedical applications / edited by Dr. Ravin Narain, Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada.
2. Hermanson, G.T. “Bioconjugate Techniques”. Academic Press 3rd edition, 2013.
3. Sam Massa and Nick Devoogdt (eds.), Bioconjugation: Methods and Protocols, Methods in Molecular Biology, vol. 2033, Springer Science+Business Media, LLC, part of Springer Nature 2019.
4. Sonny S. Mark (ed.), Bioconjugation Protocols: Strategies and Methods, Methods in Molecular Biology vol. 751, DOI 10.1007/978-1-61779-151-2_1, © Springer Science+Business Media, LLC 2011.
5. Chrostof M.Niemeyer (Eds) Methods in Molecular Biology. 283. Bioconjugation Protocols Strategies and Methods. Humana Press.