BO4313 Drug Discovery Laboratory Syllabus:
BO4313 Drug Discovery Laboratory Syllabus – Anna University PG Syllabus Regulation 2021
OBJECTIVES
To enable the students to enhance their hands-on experience in learning techniques towards discovery of new drugs and utilize this knowledge for industrial needs on.
• Different stages of drug discovery.
• Role of medicinal chemistry in drug research.
• Different techniques for drug discovery.
• Various strategies to design and develop new drug like molecules for biological targets.
SYNTHETIC METHODS FOR DRUG DISCOVERY
1. Synthesis of selected drugs involving two or more steps of synthesis and study of spectral analysis
of drug synthesized (Paracetamol, Aspirin, Fluorscein, acetanilide, etc.).
2. Determination of pharmacopoeia standards for the synthesized drugs.
3. Determination of QSAR parameters for drugs (partition co-efficient, dissociation constant, molar refractivity, etc.)
DISCOVERY OF DRUGS FROM NATURAL PRODUCTS
1. Extraction Techniques: Cold maceration, Hot Percolation and Soxhalation.
2. Evaluation of extraction Efficiency by yield calculation and TLC.
3. Fractionation : Solvent-solvent
4. Evaluation of fractionation efficiency by TLC fingerprinting.
5. Column chromatography and flash column chromatography.
6. Extraction and determination of alkaloids (caffeine acid from tea leaves).
7. To evaluate the antioxidant potential of herbal extracts using DPPH freeRadicalscavenging assay.
8. To evaluate the cytotoxic effect of herbal extracts using MTT assay.
9. To evaluate the nitric oxide (NO) modulatory effect of herbal extracts using Griess method.
10. Biotransformation study
TOTAL : 75 PERIODS
Required Equipments:
Soxhlet apparatus, rotary flash evaporator, Hot air oven, sonicator, mortar and pestle, TLC chamber, Fume hood, purification columns, micro-plate reader, UV spectrometer, centrifuge, required strains & consumables.
OUTCOME
On completion of this course students should be able to
1. Describe the process of drug discovery and development.
2. Discuss the challenges faced in each step of the drug discovery process .
3. Have gained a basic knowledge of synthetic and extraction methods used in drugdiscovery.
4. Organise information into a clear report.
5. Demonstrate their ability to work in teams and communicate scientific information effectively.
6. Perform common extraction techniques including maceration, percolation, soxalation etc.
REFERENCES
1. Foye’s Principles of Medicinal Chemistry. By David A. Williams, Thomas L.Lemke, Thomas L. Lernke, William O. Foye. Lippincott Williams& Wilkins Publishers; 7th Edition,2012.
2. Modern Methods of Plant Analysis – Peech and M. V. Tracey, 1955.
3. Natural Product Chemistry “A laboratory guide” by Raphealikan,2nd edition, 1991.
4. Phytochemistryvol I & II by Miller, Jan, Nostrant, Rein Hid, 2003.
5. Recent advances in Phytochemistry Vol. I & IV – Scilicet, Runeckles.
6. Remington: The Science and Practice of Pharmacy, 21st Edition, 2011.
7. Wilson and Gisvold’s Textbook of Organic Medicinal and Pharmaceutical Chemistry. ByJaime N. Delgado (Editor), Ole Gisvold (Editor), William A. Remers (Editor). Lippincott Williams& Wilkins Publishers; 10thEdition (August 1998) ISBN: 0397515839.1998