BY4012 Molecular Medicine Syllabus:
BY4012 Molecular Medicine Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
1 To impart knowledge on the fundamental technologies at a molecular level
2 To provide basic biology involved in technology
3 To impart knowledge on the molecular diagnostics using immunological and molecular approaches.
4 To impart knowledge on the nucleic acid and protein therapeutic agents
5 To provide knowledge on the types and evaluation of vaccines
UNIT I FUNDAMENTAL TECHNOLOGIES
Molecular cloning, Genomic Libraries, Amplification of DNA Using PCR, DNA Sequencing Technologies, Sequencing Whole Genomes, Genomics- proteomics, transcriptomics, metabolomics.
UNIT II BIOLOGY BEHIND THE TECHNOLOGY
Fundamental Concepts in Immunology: Immune response, Innate Immunity, Adaptive Immunity, Cells and tissues of the Immune System Types of Antibodies, Immunological Techniques, Genetic Basis of Disease: Chromosomal Disorders and Gene Mapping, Single-Gene Disorders, Polygenic Disorders and Gene Clustering, Immune Pathogenesis: Models of Immune System Lesions, Immune Hypersensitivity Disorders, Immunodeficiency Disorders, Microbial Pathogenesis : Bacterial Infections Viral Infections.
UNIT III MOLECULAR DIAGNOSTICS
Immunological Approaches To Detect Protein Biomarkers of Disease.: Enzyme-Linked Immunosorbent Assays, Immunoassays for Infectious Disease, Protein Arrays To Detect Polygenic Diseases, DNA-Based Approaches to Disease Diagnosis: Hybridization Probes, Padlock Probes, Allele-Specific PCR, TaqMan PCR, Real-Time PCR To Detect Infectious Disease Detection of Epigenetic Markers and SNP. Detecting RNA Signatures of Disease
UNIT IV NUCLEIC ACID AND PROTEIN THERAPEUTICS
Nucleic Acid Therapeutic Agents: Targeting Specific mRNAs and DNAs, Viral Delivery Systems, Nonviral Nucleic Acid Delivery Systems, Protein Therapeutics: Pharmaceuticals, Recombinant Antibodies, Enzymes.
UNIT V VACCINES
Overview on vaccination, Subunit Vaccines, Peptide Vaccines, Dendritic Cell Vaccines, DNA Vaccines, Attenuated Vaccines, Vector Vaccines, Systems Biology and Evaluation of Vaccines
TOTAL: 45 PERIODS
COURSE OUTCOMES:
On completion of the course, students will be able
CO1 To learn and comprehend the fundamental technologies at a molecular level
CO2 To understand the basic biology involved in technology
CO3 To understand the molecular diagnostics using immunological and molecular approaches.
CO4 To understand the nucleic acid and protein therapeutic agents
CO5 To have understanding and knowledge on the types and evaluation of vaccines
REFERENCES
1. Bernard R. Glick, Cheryl L. Patten, Terry L. Delovitch, “Medical Biotechnology”, American Society of Microbiology Press, 1st Edition, 2013.
2. Albert S., “Medical Biotechnology- Achievements, prospects and perceptions”, United nations University Press,1st Edition, 2005.
3. Godbey W. T., “An Introduction to Biotechnology: The Science, Technology and Medical Applications”,Woodhead Publishing, 1st Edition, 2014.
4. Firdos A. K., “Biotechnology in Medical Sciences”, Taylor and Francis, CRC Press, 1st Edition, 2014.