BY4006 Enzyme Engineering and Technology Syllabus:
BY4006 Enzyme Engineering and Technology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES
1 To become acquainted with the classification and mechanism of enzymes
2 To provide basic concepts of the kinetics of enzyme action
3 To impart knowledge about the immobilization of the enzymes
4 To understand the role of enzymes involved in biotransformation
5 To gain knowledge about the processes involved in enzymatic applications
UNIT I INTRODUCTION
Introduction to enzymes, Classification, Sources, Mechanism of enzyme action. Strategies of purification of enzymes, criteria of purity, molecular weight determination and characterization of enzymes, Enzymes of biological importance – Acetyl cholinesterase, angiotensin converting enzyme (ACE), ACE Inhibitors, HMG Co A reductase inhibitors, pseudo cholinesterase, 5’-nucleotidase (5NT), glucose-6-phosphate dehydrogenase (GPD), Isoforms, immuno reactive trypsinogen (IRT) and chymotrypsin; amylase isoenzymes
UNIT II KINETICS OF ENZYME ACTION
Methods for investigating the kinetics of enzyme catalyzed reactions – Initial velocity Studies, Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme activity, kinetics of inhibition. Modeling ofrate equations for single and multiple substrate reactions
UNIT III IMMOBILIZED ENZYMES
Techniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute, partition & diffusion on the kinetics of immobilized enzymes, design and configuration of immobilized enzyme reactors; applications ofimmobilized enzyme technology, Economic argument for immobilization
UNIT IV ENZYMES IN FUNCTIONAL GROUP TRANSFORMATION
Functional group interconversion using enzymes (hydrolysis reaction, oxidation/reduction reactions, C-C bond formations), Retrosynthetic biocatalysis, Chemoenzymatic synthesis of natural products. Industrial process using enzymes for production of drugs, fine chemicals and chiral intermediates, Catalytic antibodies, The design and construction of novel enzymes, artificial enzymes, Biotransformation of drugs (hydroxylation of Steroids).
UNIT V APPLICATIONS OF ENZYMES
Enzymes in organic synthesis, Enzymes as biosensors, Enzyme for environmental application, Enzymes for molecular biology research, Enzymes for analytical and diagnostic applications, Enzymes for food, pharmaceutical, tannery, textile, paper and pulp industries.
TOTAL : 45 PERIODS
COURSE OUTCOMES:
At the end of the course, the students will be able
CO1 To learn the classification, importance and mechanism of enzymes
CO2 To understand the methods for investigating the kinetics of enzyme action
CO3 To gain knowledge about the immobilization of enzymes and its applications
CO4 To develop the processes involved in functional group transformation using enzymes.
CO5 To impart knowledge on the processes involved in enzymatic applications
REFERENCES:
1. Bailey J.E., Ollis D.F. “Biochemical Engineering Fundamentals.”. McGraw Hill, 2nd Edition 1986.
2. Faber, Kurt “Biotransformations in Organic Chemistry: A Textbook.”, 5th Edition. Springer, 2008.
3. Palmer, Trevor. “Enzymes: Biochemistry, Biotechnology, Clinical Chemistry.” 2nd Edition, East West Press, 2008.
4. Blanch H.W., Clark D. S., “Biochemical Engineering”, Marcel Dekker, Inc. 2nd Edition, 1997.
5. Lee, James M., “Biochemical Engineering.” PHI, 1st Edition, 1992. Yeh W.K., Yang H.C., James R.M., “Enzyme Technologies: Metagenomics, Biocatalysis and Biosynsthesis”, Wiley- Blackwell, 1st Edition, 2010