BY4005 Advances in Molecular Pathogenesis Syllabus:
BY4005 Advances in Molecular Pathogenesis Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
1 To know about the host pathogen interaction and identifying virulence factors involved in viral
pathogenesis
2 To educate about vaccine development and functional genomic approaches in treatment of
fungal pathogenesis
3 To provide depth knowledge in clinical significance in bacterial pathogenesis treatments
4 To understand about the microbial toxins and modern molecular pathogenesis
5 To control pathogens by modern approaches.
UNIT I VIRAL PATHOGENESIS
Various pathogen types and modes of entry – Viral dissemination in the host – Viral virulence – Injury induced by virus – Host susceptibility of viral disease – Pattern of infection – Acute infection – Persistant infection – Latent infection – Slow infection – Methods for the study of pathogenesis – Foot and mouth disease virus, Pestiviruses, Arteriviruses, Blue tongue virus and Animal herpesviruses
UNIT II FUNGAL PATHOGENESIS
Innate humoral immunity to fungi – Acquired cellular immunity – Mucosal immunity – Intracellular pathogenesis of Histoplasma capsulatum – Facultative intracellular pathogen of Cryptococcus neoformans – Fungal interaction with leukocytes – Fungal vaccine development – Host defence against chronic disseminated Candidiasis – Study fungal virulence by using Genomics – Functional genomic approaches to fungal pathogenesis.
UNIT III BACTERIAL PATHOGENESIS
Epidemology and Clinical disease – Clinical course and basic immunology – In vitro models of Salmonella virulence – Antibiotic resistant Salmonella – Salmonella based vaccines – Shigella cellular models of infection – Influenza virus – Pathogenic Escherichia coli – Vibrio cholerae – Streptococcal disease – Haemophilus influenzae infection.
UNIT IV MANIPULATION OF HOST CELLS AND IMMUNE FUNCTION BY VIRAL PROTEINS
Clinical importance of understanding host defence – Interference with cytokine and Chemokine function – impairment of host mediated killing of infected cells – inhibition of apoptosis – Immunological structure of proteins – Class I and II MHC mediated antigen – Evasion from natural killer cells.
UNIT V MOLECULAR APPROACHES TO CONTROL
Classical approaches based on serotyping – Modern diagnosis based on highly conserved virulence factors, immune and DNA based techniques – New therapeutic strategies based on recent findings on molecular pathogenesis – Viral Vaccines – Immune modulators – New vaccine technology.
TOTAL: 45 PERIODS
COURSE OUTCOMES:
After completion of the course the students will be able to
CO1 Understand the host pathogen interaction and identifying virulence factors involved in viral pathogenesis
CO2 Gain about vaccine development and functional genomic approaches in treatment of fungal pathogenesis
CO3 Gain depth knowledge in clinical significance in bacterial pathogenesis treatments
CO4 Understand about the microbial toxins and modern molecular pathogenesis
CO5 Understand the methods involved in control of pathogens by modern approaches.
REFERENCES:
1. Flint, J., Enquist, L.W., Krug, “Principles of Virology: Molecular Biology, Pathogenesis and Control”, American Society of Microbiology, 2003.
2. Groismen, E.A., “Principles of Bacterial Pathogenesis”, Academic Press, 2001.
3. Gyles, C.L., Prescott, J.F., Songer, J.G. and Thoen C.O., “Pathogenesis of Bacterial Infections in Animals”, 3rd Edition, Wiley-Blackwell, 2004.
4. Mettenleitter, T.C. and Sobrino, F.,“Animal Viruses: Molecular Biology”, Caister Academic Press, 2008.
5. Norkin, L.C., “Virology: Molecular Biology and Pathogenesis”, ASM Press, 2009.
6. Madigan, Michael T. “Biology of Microorganisms”, 15th ed., 2017
7. Salyers, Abigail A. “Bacterial Pathogenesis: A Molecular Approach”, 3rd ed., 2010