BY4103 Advanced Immunology Syllabus:
BY4103 Advanced Immunology Syllabus – Anna University PG Syllabus Regulation 2021
COURSE OBJECTIVES:
To understand the structure, functions and integration of immune system
To explain the antigen-antibody interactions that offers defence mechanism
To educate the importance immunoregulation in Immunity development
To explain various techniques of therapeutically significant monoclonal and engineered
antibodies production
To educate the importance of Immuno therapeutics development for Clinical Applications
UNIT I INNATE IMMUNITY
Introduction to the Immune system – Various components of the immune system – Innate immune response – Inflammatory response. Cellular and Molecular aspects of the innate immune system- Recognition of pathogens and activation of Toll-like receptors- complement system.
UNIT II ADAPTIVE IMMUNITY
Antibody structure and functions – Antibody mediated and cell mediated immunity – components of cell-mediated immunity. Antigen-processing and presentation. MHC – structure and function. Antigen receptors and accessory molecules of T lymphocytes- B- cell development and activation, generation of B-cell diversity – Mechanism of immunoglobulin – gene arrangement and immunoglobulin superfamily. T-cell development – Generation of TCR diversity – Biology of Cytokines.
UNIT III IMMUNOREGULATION
Helper and suppressor cells, mechanism in immunity. Inflammation – mechanism and significance. Transplantation immunology- graft rejection and HLA antigens. Role of MHC and T cells. Prevention of graft rejection. Hypersensitivity- immediate and delayed types; mechanism and reactions. Vaccines – types, production and uses. Immunity to virus, bacteria and parasites- genetic control of immune response. Immunosuppression.
UNIT IV IMMUNOLOGICAL TECHNIQUES
PBMC separation from the blood; Isolation of monocytes/macrophages. Macrophage culture. Isolation of dendritic cells. Identification of lymphocytes based on CD markers; Production of monoclonal antibodies and Polyclonal antibodies. Principle and applications of immunoassays: RIA, ELISA, IRMA, ELFIA, ECLIA, DELFIA, TRIFMA, SLFIA, and western blot. Precipitation reaction – immunodiffusion, immune-electrophoresis, precipitin ring test. Agglutination tests –hemagglutination, febrile and latex agglutination- applications. Tumor Cell imaging Techniques- In vitro and In vivo cell tracking techniques; Immuno-electron microscopy, Immunofluorescence microscopy, Flurochromes; Staining techniques for live cell imaging and fixed cells; Flow cytometry
UNIT V IMMUNOTHERAPEUTICS
Recombinant Antibodies, Bispecific Antibodies, catalytic antibodies, humanized antibodies, monoclonal antibodies: Antibody-drug conjugates (ADCs), radiolabeled antibodies, immunotoxins, cancer vaccines (tumor cell vaccines), Antigen vaccines, dentritic cell vaccines. DNA vaccines, cell based therapeutics.
TOTAL: 45 PERIODS
COURSE OUTCOMES
After completion of the course the students will be able to
CO1 Understand the structure, functions and integration of immune system
CO2 Understand the antigen-antibody interactions that offers defence mechanism
CO3 Gain knowledge in importance of learning immunoregulation in Immunity development
CO4 Understand the importance of various techniques of therapeutically significant monoclonal and engineered antibodies production
CO5 Gain knowlege in Immunotherapeutics development for Clinical Applications
REFERENCES
1. Peter J. Delves., Seamus J. Martin., Dennis R. Burton., Ivan M. Roitt., “Essential Immunology”, Wiley -Blackwell; 13th Edition, 2017.
2. Kuby J., “Immunology”, WH Freeman & Co., 8th Edition, 2018.
3. Abbas, K.A., Litchman, A.H., Pober, J.S., “Cellular and Molecular Immunology”, Elsevier., 9th Edition, 2017.
4. Wilson K and Walker J., “Practical Biochemistry” , Cambridge University Press. 8th Edition, 2018
5. Weir, D.N, “Handbook of Experimental Immunology”, Wiley-Blackwell Publishers, 5th Edition 1997